Chapter 1　Introduction
　Section 1　The contents and theories in Histochemistry
　Section 2　The basic requirements of Histochemistry methods
Chapter 2　The Tissue Preparation
　Section 1　Tissue collection
　Section 2　Fixation
　Section 3　Tissue washing-up, dehydration and clearance
　Section 4　Embedding
　Section 5　Sectioning
　Section 6　Adherence and mounting
　Section 7　Buffer
Chapter 3　The Carbohydrate and its Derivatives Histochemistry
　Section 1　Classification
　Section 2　Histochemistry methods
Chapter 4　The Nucleic Acid Histochemistry
　Section 1　DNA Histochemical demonstration
　Section 2　Comparison demonstration of DNA and RNA Histochemistry
Chapter 5　The Lipid Histochemistry
　Section 1　Overview
　Section 2　Demonstration of lipids with physical methods
　Section 3　Demonstration of lipids with chemical methods
Chapter 6　Enzyme Histochemistry
　Section 1　Enzyme and its basic histochemical theory
　Section 2　Histochemistry for common enzymes
Chapter 7　The Basic Theory of Immunohistochemistry
　Section 1　Basic Immunology
　Section 2　Common markers and their detection
　Section 3　Basic conditions
Chapter 8　Common Methods Used in Immunohistochemistry
　Section 1　Principles
　Section 2　Immunofluorescence method
　Section 3　Immunoenzyme technique
　Section 4　Avidin-biotin method
　Section 5　Protein A method
　Section 6　Immunogold method and immuno gold-silver method
Chapter 9　Specificity and Sensitivity of Immunohistochemistry
　Section 1　Specificity and specific staining
　Section 2　Control experiment
　Section 3　The methods to enhance immunohistochemical sensitivity
Chapter 10　The Double-Staining in Immunohistochemistry
　Section 1　Double-staining Immunohistochemistry on serial section
　Section 2　Immunofluorescence double labeling technique
　Section 3　Immunoenzyme double staining technique
　Section 4　Immunoenzyme-Immunofluorescence double staining
　Section 5　Immunoenzyme-Immunogold double staining
Chapter 11　The Lectin Histochemistry
　Section 1　Characteristics and application of lectin
　Section 2　Application of lectin Histochemistry
Chapter 12　The Progress of in Situ Display
　Section 1　The envision method
　Section 2　The catalyzed signal amplification method
　Section 3　The ferric oxide alternative method for HRP
Chapter 13　In Situ Hybridization Immunohistochemistry
　Section 1　Basic principle
　Section 2　Probe preparation
　Section 3　Procedure of in situ hybridization histochemistry
　Section 4　Factors affecting in situ hybridization
　Section 5　Control test
Chapter 14　In Situ Polymerase Chain Reaction Technique
　Section 1　Basic principle
　Section 2　Basic types
　Section 3　Procedure
　Section 4　Application of in situ PeR technology
Chapter 15　Electron Microscopic Cytochemical Technique
　Section 1　Electron microscopic enzyme cytochemistry technology
　Section 2　Electron microscopic Immunocytochemical technique
Chapter 16　The Quantitative Assay of Histochemistry Results
　Section 1　The photomicrography
　Section 2　The image analyzer
　Section 3　The flow cytometry
　Section 4　The laser scanning confocal microscopy
References
Appendix I　Preparation of Commonly Used Buffer
Appendix II　Histochemistry and Immunohistochemistry Experiments
Appendix III　Figures and pictures